The PAK1 Polyclonal Antibody (CAB0809) is a key tool for researchers studying PAK1, a protein involved in multiple cellular processes such as cell proliferation, migration, and invasion. This antibody is produced in rabbits and exhibits high reactivity with human samples, making it suitable for use in Western blotting and immunohistochemistry applications. PAK1, a serine/threonine kinase, is known for its role in signaling pathways that regulate cell morphology and motility. Dysregulation of PAK1 activity has been linked to various diseases, including cancer and neurological disorders.
By targeting PAK1 with this antibody, researchers can gain insights into the function and localization of this important protein in different cell types. Studies on PAK1 are essential for understanding its involvement in various diseases and for developing targeted therapies that can modulate its activity. This antibody provides a valuable tool for investigating the role of PAK1 in cellular processes and disease pathogenesis.
Product Name:
PAK1 Rabbit Polyclonal Antibody
SKU:
CAB0809
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 130-250 of human PAK1 (NP_001122092.1).
This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Mutations in this gene have been associated with macrocephaly, seizures, and speech delay. Overexpression of this gene is also reported in many cancer types, and particularly in breast cancer. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
5058
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PAK1 antibody (CAB0809) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
