The PAIP1 Polyclonal Antibody (CAB6042) is a valuable tool for research involving PAIP1, a protein involved in mRNA translation and stability regulation. This antibody, raised in rabbits, is highly specific and reactive towards human samples, making it ideal for use in Western blot applications. By binding to the PAIP1 protein, this antibody enables researchers to detect and analyze PAIP1 expression in various cell types, offering insights into its functions in cellular processes.PAIP1 plays a crucial role in the regulation of protein synthesis by facilitating the interaction between poly(A)-binding protein (PABP) and eukaryotic translation initiation factor 4G (eIF4G). This interaction is essential for the translation of mRNA into proteins, highlighting the significance of PAIP1 in cellular function and homeostasis.
Research on PAIP1 is particularly relevant in understanding processes such as cell growth, proliferation, and differentiation, as well as in diseases like cancer where dysregulation of translation mechanisms is common.By studying the role of PAIP1 using this antibody, researchers can gain valuable insights into the molecular mechanisms underlying protein synthesis and potentially uncover new targets for therapeutic interventions in cancer and other related disorders. This antibody is a reliable tool for investigating the function of PAIP1 and its implications in various biological processes, contributing to advancements in molecular biology and drug discovery efforts.
Product Name:
PAIP1 Rabbit Polyclonal Antibody
SKU:
CAB6042
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PAIP1 (NP_877590.1).
The protein encoded by this gene interacts with poly(A)-binding protein and with the cap-binding complex eIF4A. It is involved in translational initiation and protein biosynthesis. Overexpression of this gene in COS7 cells stimulates translation. Alternative splicing occurs at this locus and three transcript variants encoding three distinct isoforms have been identified.
Purification Method:
Affinity purification
Gene ID:
10605
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PAIP1 antibody (CAB6042) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
