The PAH Polyclonal Antibody (CAB1559) is specifically developed for research involving PAH, a critical enzyme involved in the metabolism of phenylalanine. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to the PAH protein, this antibody enables accurate detection and analysis in various cell types, making it an ideal tool for studies in metabolic disorders and related research.PAH, also known as phenylalanine hydroxylase, plays a crucial role in the breakdown of phenylalanine, an essential amino acid. Mutations in the PAH gene can lead to phenylketonuria (PKU), a metabolic disorder that affects the body's ability to process phenylalanine.
Research on PAH is instrumental in understanding the mechanisms underlying PKU and developing potential treatments for this genetic condition.Investigating the function and regulation of PAH is essential for advancing our knowledge of metabolic disorders and developing targeted therapies for individuals affected by conditions like PKU. The PAH Polyclonal Antibody (CAB1559) offers researchers a valuable tool for studying PAH in various biological contexts, contributing to the advancement of precision medicine and personalized treatments for metabolic disorders.
Product Name:
PAH Rabbit Polyclonal Antibody
SKU:
CAB1559
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human PAH (NP_000268.1).
This gene encodes a member of the biopterin-dependent aromatic amino acid hydroxylase protein family. The encoded phenylalanine hydroxylase enzyme hydroxylates phenylalanine to tyrosine and is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.
Purification Method:
Affinity purification
Gene ID:
5053
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of HepG2, using PAH antibody (CAB1559) at 1:700 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
