Infectious Disease ELISA
Infectious Disease ELISA Kits
Quantitative and Semi-quantitative kits for Infectious Disease detection in humans & animals

Why choose Infectious Disease ELISA?

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"Assay Genie provided a critical ELISA kit that is very difficult to find on the market. Not only did this kit work dependably well, but it also provided me with the measurement sensitivity that I needed to evaluate my samples."
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Detection Principle & Protocol
An ELISA protocol involves coating a microplate with a specific capture antibody, adding the sample containing the target analyte, and incubating to allow binding. After washing to remove unbound substances, a detection antibody (linked to an enzyme) is added, followed by a substrate solution that produces a measurable color change. The optical density (OD) is read using a spectrophotometer, with absorbance values correlating to analyte concentration.

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Why is important to monitor Infectious Diseases?

- Early Detection and Containment | Helps identify outbreaks early, allowing for rapid response and containment.
- Disease Adaptation and Evolution | Tracking pathogens reveals how they evolve, adapt to new hosts, and develop resistance to treatments.
- Managing Epidemic and Endemic Diseases | Helps differentiate between emerging outbreaks and persistent regional diseases.
- Preventing Future Outbreaks | Continuous monitoring enables proactive measures to mitigate health threats.
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Frequently asked questions
Possible Cause | Possible Solution |
Reagents not at room temperature | All reagents should at room temperature from the start of the assay. Room temperature should be reached following 15–20 minutes on the bench. |
Incubation time too short | Follow manufacturer guidelines in the technical manuals |
Incorrect wavelength | Manufactured kits have optimized protocols. Make sure to use recommended wavelength. Ensure plate reader is set accurately for type of substrate being used |
Target present below detection limits of assay | Decrease dilution factor or concentrate samples |
Reagents are poorly mixed, the standard has degraded or pipetting errors.
For more troubleshooting suggestions vist our 101 ELISA Troubleshooting Tips!