The PABPN1 Rabbit Monoclonal Antibody (CAB1735) is a highly specific antibody designed for research involving PABPN1, a key protein involved in RNA metabolism and linked to muscular dystrophy. This antibody, generated in rabbits, shows high reactivity with human samples and is validated for use in various applications, including Western blot and immunofluorescence.PABPN1, also known as poly(A) binding protein nuclear 1, plays a crucial role in RNA processing and stability, particularly in the regulation of polyadenylation. Dysregulation of PABPN1 has been implicated in diseases such as oculopharyngeal muscular dystrophy (OPMD), highlighting its importance in physiological processes and disease development.
This antibody specifically binds to the PABPN1 protein, allowing for its detection and analysis in different cell types and tissues. Its high specificity and sensitivity make it an ideal tool for studying the function of PABPN1 in RNA metabolism, muscle biology, and related disorders. Researchers studying neuromuscular diseases, RNA processing, and gene regulation will find this antibody invaluable for their experiments.
Product Name:
PABPN1 Rabbit Monoclonal Antibody
SKU:
CAB1735
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PABPN1 (Q86U42).
This gene encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails at the 3' ends of eukaryotic transcripts and controls the size of the poly(A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly(A) binding protein is localized in the cytoplasm. This gene contains a GCG trinucleotide repeat at the 5' end of the coding region, and expansion of this repeat from the normal 6 copies to 8-13 copies leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Related pseudogenes have been identified on chromosomes 19 and X. Read-through transcription also exists between this gene and the neighboring upstream BCL2-like 2 (BCL2L2) gene.
Purification Method:
Affinity purification
Gene ID:
8106
Clone Number:
ARC0730
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using PABPN1 Rabbit mAb (CAB1735) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
