The PABPN1 Polyclonal Antibody (CAB6041) is a key tool for researchers studying the PABPN1 protein, which plays a crucial role in mRNA processing and stability. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the PABPN1 protein, researchers can accurately detect and analyze its expression in a variety of cell types, making it an essential tool for studies in molecular biology and disease research.PABPN1 is involved in regulating gene expression at the post-transcriptional level, making it a critical player in processes like mRNA polyadenylation and nuclear export.
Dysregulation of PABPN1 has been linked to various diseases, including muscular dystrophy and cancer, highlighting its importance in cellular function and disease pathology. By investigating the role of PABPN1, researchers can gain valuable insights into the underlying mechanisms of these disorders and potentially identify novel therapeutic targets for treatment.
Product Name:
PABPN1 Rabbit Polyclonal Antibody
SKU:
CAB6041
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PABPN1 (NP_004634.1).
B cells,HeLa,HepG2,U-87MG,Mouse brain,Mouse testis
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
33kDa
Observed MW:
45kDa
This gene encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails at the 3' ends of eukaryotic transcripts and controls the size of the poly(A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly(A) binding protein is localized in the cytoplasm. This gene contains a GCG trinucleotide repeat at the 5' end of the coding region, and expansion of this repeat from the normal 6 copies to 8-13 copies leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Related pseudogenes have been identified on chromosomes 19 and X. Read-through transcription also exists between this gene and the neighboring upstream BCL2-like 2 (BCL2L2) gene.
Purification Method:
Affinity purification
Gene ID:
8106
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PABPN1 antibody (CAB6041) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
