The PABPC4 Polyclonal Antibody (CAB5948) is a high-quality antibody designed for research involving PABPC4, a protein involved in mRNA metabolism and translation regulation. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications. It binds to the PABPC4 protein, allowing for the detection and analysis of PABPC4 levels in various cell types.PABPC4, also known as Poly(A) Binding Protein Cytoplasmic 4, plays a crucial role in post-transcriptional regulation of gene expression by binding to the poly(A) tail of mRNA. Its involvement in mRNA stability and translation initiation makes it a key player in various cellular processes.
Research into PABPC4 can provide insights into gene expression regulation and its dysregulation in diseases such as cancer and neurological disorders.By using the PABPC4 Polyclonal Antibody (CAB5948), researchers can better understand the function and regulation of PABPC4 in different biological contexts, paving the way for potential therapeutic interventions targeting this protein. Its high specificity and reactivity make it a valuable tool for studies in molecular biology, cancer research, and other fields aiming to unravel the complexities of gene expression regulation.
Product Name:
PABPC4 Rabbit Polyclonal Antibody
SKU:
CAB5948
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PABPC4 (NP_003810.1).
Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. This protein has also been found to interact with coronavirus nucleocapsid proteins and is thought to inhibit coronavirus replication.
Purification Method:
Affinity purification
Gene ID:
8761
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using PABPC4 Rabbit pAb (CAB5948) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
