The p53 Polyclonal Antibody (CAB21630) is a valuable tool for research involving the p53 protein, a tumor suppressor that plays a crucial role in regulating cell growth and preventing cancer development. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been extensively validated for use in Western blot applications.p53, also known as the "guardian of the genome," is involved in various cellular processes such as DNA repair, cell cycle control, and apoptosis. Mutations in the p53 gene are common in many types of cancer, underscoring the importance of studying its function and regulation.
The p53 Polyclonal Antibody enables precise detection and quantification of the p53 protein in different cell types, making it an ideal choice for research in oncology and molecular biology.By utilizing this antibody, researchers can gain insights into the mechanisms underlying p53-mediated tumor suppression and explore potential therapeutic strategies that target the p53 pathway. Understanding the intricate role of p53 in cancer biology is essential for developing effective treatments and improving patient outcomes.
Product Name:
p53 Polyclonal Antibody
SKU:
CAB21630
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 290-390 of mouse p53 (NP_035770.2).
This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mice deficient for this gene are developmentally normal but are susceptible to spontaneous tumors. Evidence to date shows that this gene contains one promoter, in contrast to alternative promoters of the human gene, and transcribes a few of splice variants which encode different isoforms, although the biological validity or the full-length nature of some variants has not been determined.
Purification Method:
Affinity purification
Gene ID:
22059
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using p53 Rabbit pAb (CAB21630) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
