The p53 Polyclonal Antibody (CAB21629) is a valuable tool for researchers studying the tumor suppressor protein p53. This antibody, generated in rabbits, is highly specific to human samples and is validated for use in Western blot applications. By binding to p53 protein, this antibody allows for the detection and analysis of p53 in various cell types, making it ideal for investigations in cancer biology and related fields.p53, also known as the "guardian of the genome," plays a crucial role in regulating cell growth and responding to DNA damage. Mutations in the p53 gene are commonly associated with cancer development, making this protein a key target for understanding tumor formation and progression.
Research on p53 activity is essential for developing targeted therapies for cancer treatment and improving patient outcomes.In conclusion, the p53 Polyclonal Antibody (CAB21629) is a reliable tool for researchers interested in exploring the function of p53 in cancer biology and beyond. Its high specificity and sensitivity make it a valuable asset for studies aiming to uncover the molecular mechanisms underlying tumor development and potential therapeutic interventions.
Product Name:
p53 Polyclonal Antibody
SKU:
CAB21629
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-393 of human p53. (NP_000537.3).
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Purification Method:
Affinity purification
Gene ID:
7157
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using p53 Rabbit pAb (CAB21629) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
