The P4HB Polyclonal Antibody is a specialized research tool designed for studying P4HB, a key protein involved in various cellular processes such as protein folding and redox reactions. This antibody is produced in rabbits and exhibits high reactivity with human samples, making it an excellent choice for Western blot applications. By binding specifically to the P4HB protein, researchers can accurately detect and analyze this crucial molecule in different cell types, offering valuable insights into its role in cellular function.P4HB, also known as protein disulfide-isomerase, plays a critical role in maintaining protein structure and function through its involvement in disulfide bond formation and isomerization.
Dysregulation of P4HB has been linked to various diseases, including cancer, neurodegenerative disorders, and cardiovascular conditions. By studying the activity of P4HB, researchers can uncover new therapeutic targets and potential treatment strategies for these diseases.The P4HB Polyclonal Antibody is a valuable tool for scientists conducting research in cell biology, biochemistry, and disease pathology. Its specificity and sensitivity make it a reliable choice for detecting P4HB in various experimental settings, providing essential data for understanding the molecular mechanisms underlying cellular processes and disease development.
Product Name:
P4HB Polyclonal Antibody
SKU:
CAB0709
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 329-508 of human P4HB (NP_000909.2).
This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.
Purification Method:
Affinity purification
Gene ID:
5034
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using P4HB Rabbit pAb (CAB0709) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
