P4HB Polyclonal Antibody (CAB0709)
- SKU:
- CAB0709
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
- IHC
- IF
Description
Product Name: | P4HB Polyclonal Antibody |
SKU: | CAB0709 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 329-508 of human P4HB (NP_000909.2). |
Sequence: | PESE ELTA ERIT EFCH RFLE GKIK PHLM SQEL PEDW DKQP VKVL VGKN FEDV AFDE KKNV FVEF YAPW CGHC KQLA PIWD KLGE TYKD HENI VIAK MDST ANEV EAVK VHSF PTLK FFPA SADR TVID YNGE RTLD GFKK FLES GGQD GAGD DDDL EDLE EAEE PDME EDDD QKAV KDEL |
Tested Applications: | WB IHC-P IF/ICC ELISA |
Recommended Dilution: | WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 |
Synonyms: | DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; CLCRP1; ERBA2L; P4Hbeta; P4HB |
Positive Sample: | HeLa,293T,HepG2,NIH/3T3,Mouse liver,Rat liver |
Conjugate: | Unconjugated |
Cellular Localization: | Cell membrane, Endoplasmic reticulum, Endoplasmic reticulum lumen, Melanosome, Peripheral membrane protein. |
Calculated MW: | 57kDa |
Observed MW: | 57kDa |
This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.
Purification Method: | Affinity purification |
Gene ID: | 5034 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |