The P4HA3 Polyclonal Antibody (CAB13767) is a crucial tool for research involving P4HA3, a key enzyme involved in collagen synthesis and tumor growth. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been specifically validated for use in Western blot applications. By targeting the P4HA3 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential asset for studies focusing on cancer biology and extracellular matrix remodeling.
P4HA3, a member of the prolyl 4-hydroxylase enzyme family, plays a critical role in modifying collagen structure and promoting tumor invasion and metastasis. Its significance in tumor progression and metastatic spread make it a promising target for research aimed at developing novel therapeutic strategies for cancer treatment. Understanding the function of P4HA3 is essential for uncovering potential biomarkers and therapeutic targets that could improve cancer diagnosis and patient outcomes.
Product Name:
P4HA3 Rabbit Polyclonal Antibody
SKU:
CAB13767
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-110 of human P4HA3 (NP_878907.1).
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
283208
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of 293T cells, using P4HA3 antibody (CAB13767) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.