The P4HA2 Polyclonal Antibody (CAB4262) is a valuable tool for researchers studying P4HA2, an enzyme involved in collagen synthesis and tumor progression. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the P4HA2 protein, this antibody allows for the detection and analysis of P4HA2 expression in various cell types, making it an essential component of studies in cancer research and tissue remodeling.P4HA2, also known as prolyl 4-hydroxylase subunit alpha 2, plays a crucial role in stabilizing the triple helical structure of collagen, a key component of the extracellular matrix.
Aberrant expression of P4HA2 has been implicated in tumor growth, invasion, and metastasis, highlighting its significance as a potential therapeutic target in cancer. By understanding the function of P4HA2, researchers can uncover new insights into the molecular mechanisms driving tumorigenesis and potentially develop novel strategies for cancer treatment.
Product Name:
P4HA2 Rabbit Polyclonal Antibody
SKU:
CAB4262
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 239-333 of human P4HA2 (NP_004190.1).
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
8974
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using P4HA2 antibody (CAB4262) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
