The P4HA1 Polyclonal Antibody (CAB10538) is a valuable tool for researchers studying P4HA1, a key enzyme involved in collagen synthesis and tumor progression. This antibody is produced in rabbits and is highly specific for detecting P4HA1 in human samples. Validated for use in Western blot applications, this antibody allows for the precise detection and analysis of P4HA1 protein levels in various cell types.P4HA1 plays a crucial role in the formation of stable collagen structures, making it a significant factor in the development and progression of cancer.
By targeting P4HA1, researchers can gain insights into the mechanisms driving tumor growth and metastasis, leading to potential therapeutic interventions in cancer treatment.With its high reactivity and specificity, the P4HA1 Polyclonal Antibody is an invaluable tool for studies in oncology and collagen biology. Its ability to detect and quantify P4HA1 protein expression makes it a valuable asset in advancing our understanding of collagen metabolism and its implications in disease.
Product Name:
P4HA1 Rabbit Polyclonal Antibody
SKU:
CAB10538
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 235-534 of human P4HA1 (NP_001017962.1).
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
5033
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using P4HA1 antibody (CAB10538) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
