Anti-P2RX4 Antibody (CAB6682)

Antibody Name:	Anti-P2RX4 Antibody
Antibody SKU:	CAB6682
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 55-338 of human P2RX4 (NP_002551.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	22Rv1, Mouse kidney, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 55-338 of human P2RX4 (NP_002551.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QETD SVVS SVTT KVKG VAVT NTSK LGFR IWDV ADYV IPAQ EENS LFVM TNVI LTMN QTQG LCPE IPDA TTVC KSDA SCTA GSAG THSN GVST GRCV AFNG SVKT CEVA AWCP VEDD THVP QPAF LKAA ENFT LLVK NNIW YPKF NFSK RNIL PNIT TTYL KSCI YDAK TDPF CPIF RLGK IVEN AGHS FQDM AVEG GIMG IQVN WDCN LDRA ASLC LPRY SFRR LDTR DVEH NVSP GYNF RFAK YYRD LAGN EQRT LIKA YGIR FDII VFGK AGKF DIIP TMIN
Gene ID:	5025
Uniprot:	Q99571
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	40kDa/43kDa/45kDa
Observed MW:	62kDa

Synonyms:

P2RX4, P2X4, P2X4R

Background:

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants, some protein-coding and some not protein-coding, have been found for this gene.

UniProt Protein Function:	P2X4: Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin. Belongs to the P2X receptor family.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Channel, ligand-gated; Membrane protein, integral Chromosomal Location of Human Ortholog: 12q24.32 Cellular Component: membrane; cell soma; perinuclear region of cytoplasm; apical part of cell; lysosomal membrane; integral to plasma membrane; postsynaptic density; dendritic spine; plasma membrane; terminal button; integral to nuclear inner membrane; cell junction Molecular Function:protein homodimerization activity; copper ion binding; zinc ion binding; cadherin binding; drug binding; ATP binding; purinergic nucleotide receptor activity; ATP-gated cation channel activity; receptor binding Biological Process: positive regulation of nitric oxide biosynthetic process; generation of action potential; positive regulation of calcium-mediated signaling; regulation of sodium ion transport; sensory perception of pain; signal transduction; vasodilation; response to ATP; endothelial cell activation; membrane depolarization; transport; calcium ion transport; regulation of blood pressure; positive regulation of prostaglandin secretion; tissue homeostasis; regulation of excitatory postsynaptic membrane potential; positive regulation of calcium ion transport; nitric oxide biosynthetic process; protein homooligomerization
NCBI Summary:	The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants, some protein-coding and some not protein-coding, have been found for this gene. [provided by RefSeq, Feb 2012]
UniProt Code:	Q99571
NCBI GenInfo Identifier:	116242696
NCBI Gene ID:	5025
NCBI Accession:	Q99571.2
UniProt Related Accession:	Q99571
Molecular Weight:	Predicted Molecular Mass: 33.2kDaAccurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
NCBI Full Name:	P2X purinoceptor 4
NCBI Synonym Full Names:	purinergic receptor P2X 4
NCBI Official Symbol:	P2RX4
NCBI Official Synonym Symbols:	P2X4; P2X4R
NCBI Protein Information:	P2X purinoceptor 4
UniProt Protein Name:	P2X purinoceptor 4
UniProt Synonym Protein Names:	ATP receptor; Purinergic receptor
Protein Family:	P2X purinoceptor
UniProt Gene Name:	P2RX4
UniProt Entry Name:	P2RX4_HUMAN

	Western blot analysis of extracts of various cell lines, using P2RX4 antibody (CAB6682) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of MCF7 cells using P2RX4 antibody (CAB6682). Blue: DAPI for nuclear staining.

Antibodies

P2RX4 Antibody (PACO11037)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)