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Oxaloacetate Colorimetric Assay Kit

SKU:
MAES0273
Product Type:
Assay
Instrument:
Microplate Reader
Detection Method:
Colorimetric
Sample Type:
Animal Tissue
Sensitivity:
0.017 mmol/L
Range:
0.017-1 mmol/L
Assay Time:
15 min
€589
Frequently bought together:

Description

Product Name:Oxaloacetate Colorimetric Assay Kit
Product Code:MAES0273
Sample Type:Animal Tissue
Assay Time:15 min
Assay Type:Quantitative
Detection Instrument:Microplate reader (402-422 nm, optimum wavelength: 412 nm)
Detection Method:Colorimetric method
Sensitivity:0.017 mmol/L
Detection Range:0.017-1 mmol/L
Inter-assay CV:0.023
Intra-assay CV:0.021

Oxaloacetic acid (OAA), one of the intermediates in the tricarboxylic acid cycle is an important substance in carbon and nitrogen metabolism. Oxaloacetate reacts with substrate acetyl-coA under the action of enzyme. The substance produced can react with DTNB, which has the maximum absorption peak at 412 nm. The content of oxaloacetate can be determined by measuring the absorbance value.

Storage:-20°C
Valid Period:12 months
Other Reagents Required:Incubator, Ultrafiltration tube (50kD)
Other Instruments Required:Normal saline (0.9% NaCl)

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