Sheep IL-8 DIY ELISA Kit (KES0107)
- SKU:
- KES0107
- Product Type:
- ELISA Kit
- Reactivity:
- Sheep
- Applications:
- ELISA
- ELISA Type:
- DIY ELISA
- Size:
- 10 Plates
- Synonyms:
- CXCL8, GCP-1, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1
Description
Sheep IL-8 DIY ELISA Kit
The Ovine IL-8 ELISA Kit from AssayGenie is specifically designed for the precise measurement of Interleukin-8 levels in ovine serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Interleukin-8, also known as CXCL8, is a key cytokine involved in inflammation and immune responses. It plays a critical role in the recruitment of neutrophils to sites of infection or injury, as well as promoting angiogenesis and tumor growth.
Monitoring IL-8 levels can provide valuable insights into the development and progression of various diseases, including inflammatory disorders, cancer, and infectious diseases.With the Ovine IL-8 ELISA Kit, researchers can effectively study the role of IL-8 in different pathophysiological conditions and explore potential therapeutic interventions. Trust AssayGenie for reliable and accurate measurement of IL-8 levels in ovine samples, advancing your research efforts in immunology, oncology, and beyond.
| Product Name: | Sheep IL-8 DIY ELISA |
| Product Code: | KES0107 |
| Species: | Sheep |
| Target: | CXCL8 |
| Synonyms: | CXCL8, GCP-1, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1 |
| Application: | ELISA |
| ELISA Type: | DIY ELISA Kit |
| Size: | 10 Plates |
| Shipping: | Room temperature |
| Storage: | Stable for up to twelve months from date of receipt at 2-8°C. |
| Description | Usage | Quantity |
| Anti-Sheep IL-8 Polyclonal Antibody | Capture Antibody | 100 µg |
| Biotinylated Anti-Sheep IL-8 Polyclonal Antibody | Detection Antibody | 50 µg |
| Sheep IL-8 Recombinant Protein | Standard | 5 µg |
| Reagent | Suggested Formulation |
| DPBS: | 0.008M sodium phosphate, 0.002M potassium phosphate, 0.14M sodium chloride, 0.01M potassium chloride, pH 7.4 |
| 96-well ELISA Plate: | Clear, flat-bottom, high-binding 96-well plate, 8-wells per strip, 350 µL per well (ELISA Plates: KESAP003) |
| Standard and Sample Diluent: | The optimal Standard and Sample Diluent will need to be determined for each sample type to obtain optimal recovery and linearity. The appropriate Standard and Sample Diluent will mimic the sample's response to a known quantity of protein standard and will provide linear results when diluted. Often a 1:4 dilution of the sample in Reagent Diluent will provide acceptable recovery and linearity. |
| Reagent Diluent and Blocking Buffer: | 4% BSA in DPBS, 0.2 µm filtered |
| Wash Buffer: | 0.05% Tween®-20 in DPBS |
| Streptavidin-HRP: | Enzymatic reagent to react with biotinylated detection antibody (Streptavidin-HRP: KESAP002) |
| Substrate: | 3,3',5,5'-tetramethylbenzidine (TMB) Substrate (ELISA Accessory Pack: KESAP001) |
| Stop Solution: | 0.18 M Sulfuric Acid (ELISA Accessory Pack: KESAP001) |
| Plate Sealer: | Adhesive film to prevent evaporation |
*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1. | Prepare Capture Antibody in DPBS at desired working concentration. |
| 2. | Add 100 µL of Capture Antibody Working Solution to appropriate wells. |
| 3. | Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 12-24 hours. |
| 4. | Empty Capture Antibody Working Solution from plate. Blot plate onto paper towels or other absorbent material. |
| 5. | Add 100 µL of Blocking Buffer to appropriate wells. |
| 6. | Cover plate with Plate Sealer and incubate at room temperature for 1-3 hours. |
| 7. | Empty Blocking Buffer from plate. Blot plate onto paper towels or other absorbent material. |
| 8. | Prepare Standard and sample as desired with Standard and Sample Diluent. |
| 9. | Add 100 µL of Standard or sample to appropriate wells. Note: Run each Standard or sample in duplicate. |
| 10. | Cover plate with Plate Sealer and incubate at room temperature for 1 hour. |
| 11. | Wash plate FOUR times with Wash Buffer. Note: Gently squeeze the long sides of plate frame before washing to ensure all strips remain securely in the frame. Empty plate contents. Use a squirt wash bottle to vigorously fill each well completely with 1X Wash Buffer, then empty plate contents. Repeat procedure three additional times for a total of FOUR washes. Blot plate onto paper towels or other absorbent material. |
| 12. | Prepare Detection Antibody in Reagent Diluent at desired working concentration. |
| 13. | Add 100 µL of Detection Antibody Working Solution to each well. |
| 14. | Cover plate with Plate Sealer and incubate at room temperature for 1 hour. |
| 15. | Wash plate FOUR times with Wash Buffer as described in step 11. |
| 16. | Prepare Streptavidin-HRP in Reagent Diluent at desired working concentration. |
| 17. | Add 100 µL of Streptavidin-HRP Working Solution to each well. |
| 18. | Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. |
| 19. | Wash plate FOUR times with Wash Buffer as described in step 11. |
| 20. | Add 100 µL of TMB Substrate Solution to each well. |
| 21. | Develop the plate in the dark at room temperature for 30 minutes or as desired. Note: Do NOT cover plate with Plate Sealer. |
| 22. | Stop reaction by adding 100 µL of Stop Solution to each well. |
| 23. | Measure absorbance on a plate reader at 450 nm. |
The Sheep IL-8 DIY ELISA kit from Assay Genie can assay for CXCL8 in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues. Sheep IL-8 DIY ELISA Kit from Assay Genie allows researchers to develop their own ELISA plates for CXCL8 using our unique combination of capture and detection antibodies.
Each Sheep IL-8 DIY ELISA Kit contains capture antibody, standard, and detection antibody for development of an CXCL8 ELISA. CXCL8 antibodies in this kit have been determined to function in an ELISA with the CXCL8 standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined and require optimisation for the development of this kit. A working knowledge of ELISA is strongly recommended.
Related Products
