The OTUB1 Polyclonal Antibody (CAB11656) is designed for research involving OTUB1, a protein known for its role in regulating various cellular processes, particularly in the context of ubiquitination and DNA damage response. The antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it a reliable tool for experimental studies.OTUB1, a deubiquitinating enzyme, is involved in the removal of ubiquitin molecules from target proteins, thereby influencing their stability and function. Its implications in DNA damage repair pathways highlight its importance in maintaining genomic integrity and cell survival.
Researchers can utilize this antibody in Western blot analyses to detect and quantify OTUB1 levels in different cell types, aiding in the exploration of its functions in various biological processes.The versatile nature of OTUB1 in cellular signaling pathways makes it a promising target for investigations in cancer biology, molecular biology, and drug development. By elucidating the mechanisms by which OTUB1 contributes to cellular homeostasis and disease progression, researchers can pave the way for innovative therapeutic strategies targeting ubiquitin-mediated pathways.
Product Name:
OTUB1 Rabbit Polyclonal Antibody
SKU:
CAB11656
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human OTUB1 (NP_060140.2).
The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
55611
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using OTUB1 Rabbit pAb (CAB11656) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
