The OTUB1 Polyclonal Antibody (CAB10313) is a research tool designed for the study of OTUB1, a key enzyme involved in the regulation of protein stability and immune response. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By targeting the OTUB1 protein, researchers can accurately detect and analyze its expression in various cell types, making it an essential tool for investigations in immunology and cancer research.
OTUB1 is known to play a crucial role in the immune system by regulating the stability of proteins involved in immune response pathways. Dysfunction of OTUB1 has been linked to various diseases, including cancer and autoimmune disorders, highlighting its potential as a therapeutic target. By studying the function of OTUB1, researchers can gain insights into the mechanisms underlying these diseases and develop novel treatment strategies aimed at modulating immune responses for therapeutic benefits.
Product Name:
OTUB1 Rabbit Polyclonal Antibody
SKU:
CAB10313
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human OTUB1 (NP_060140.2).
The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
55611
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using OTUB1 antibody (CAB10313) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
