The OTC Polyclonal Antibody (CAB9834) offered by AssayGenie is a high-quality research tool for studying OTC, a key enzyme involved in the urea cycle. This antibody, produced in rabbits, is specifically designed for use in Western blot applications and has been validated for its reactivity with human samples.OTC, or Ornithine Transcarbamylase, plays a crucial role in the detoxification of ammonia in the body by converting ornithine and carbamoyl phosphate into citrulline. Dysregulation of OTC can lead to hyperammonemia, a serious condition that can result in neurological damage or death.
Therefore, studying the expression and activity of OTC is essential for understanding and potentially treating disorders related to ammonia metabolism.By using the OTC Polyclonal Antibody (CAB9834), researchers can accurately detect and analyze OTC protein levels in various cell types, providing valuable insights into its function and potential therapeutic targets. This antibody is an excellent tool for investigations in fields such as biochemistry, molecular biology, and metabolic disorders. Enhance your research with the OTC Polyclonal Antibody from AssayGenie.
Product Name:
OTC Rabbit Polyclonal Antibody
SKU:
CAB9834
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 215-354 of human OTC (NP_000522.3).
This nuclear gene encodes a mitochondrial matrix enzyme. The encoded protein is involved in the urea cycle which functions to detoxify ammonia into urea for excretion. Mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia.
Purification Method:
Affinity purification
Gene ID:
5009
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with OTC using OTC Rabbit pAb (CAB9834) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
