The OT (Oxytocin) ELISA Kit is specifically designed for the precise quantification of oxytocin levels in human serum, plasma, and tissue culture supernatants. This kit offers exceptional sensitivity and accuracy, guaranteeing consistent and dependable results, which makes it perfect for various research purposes.Oxytocin is a critical hormone involved in social bonding, maternal behavior, and stress regulation. It plays a vital role in various physiological processes, including childbirth, lactation, and emotional responses.
Oxytocin has also been implicated in mental health disorders, autism, and bonding behaviors, making it a valuable biomarker for investigating these conditions and exploring potential treatments.To learn more about the OT (Oxytocin) ELISA Kit, please visit: www.assaygenie.com/ot-oxytocin-elisa-kit-unes00019/
Product Name:
OT (Oxytocin) ELISA Kit (UNES00019)
SKU:
UNES00019
Target:
OT (Oxytocin) (UNES00019)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with OT. During the reaction, OT in the sample or standard competes with a fixed amount of OT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to OT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of OT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-97
94-110
93-107
Average (%)
92
101
100
1:4
Range (%)
88-100
91-108
94-108
Average (%)
94
99
101
1:8
Range (%)
80-92
92-106
98-112
Average (%)
87
97
103
1:16
Range (%)
90-103
97-110
96-108
Average (%)
95
102
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
98
EDTA plasma (n=5)
92-104
99
Cell culture media (n=5)
88-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.2
153.88
495.39
56.27
139.06
544.58
Standard deviation
2.78
7.02
16.1
2.97
6.04
23.85
C V (%)
5.23
4.56
3.25
5.28
4.34
4.38
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of OT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes OT in samples. No significant cross-reactivity or interference between OT and analogues was observed.
