The Orexin Polyclonal Antibody (CAB9821) is a valuable tool for researchers studying orexin, a neuropeptide involved in regulating sleep, arousal, and appetite. This antibody, produced in rabbits, exhibits high specificity and sensitivity when used with human samples, making it ideal for experiments in Western blot applications. By targeting the orexin protein, this antibody enables precise detection and analysis in various cell types, facilitating research in the fields of neuroscience, sleep disorders, and metabolism.
Orexin, also known as hypocretin, plays a crucial role in the regulation of wakefulness and has been implicated in disorders such as narcolepsy and insomnia. Understanding the functions and mechanisms of orexin is essential for developing effective treatments for sleep-related conditions and metabolic disorders. The Orexin Polyclonal Antibody provides researchers with a reliable tool for investigating the role of orexin in health and disease, paving the way for advancements in sleep medicine and metabolic research.
Product Name:
HCRT Rabbit Polyclonal Antibody
SKU:
CAB9821
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 34-66 of human HCRT (NP_001515.1).
This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis.
Purification Method:
Affinity purification
Gene ID:
3060
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using HCRT antibody (CAB9821) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
