The ORC2 Polyclonal Antibody (CAB15697) is a valuable tool for researchers studying ORC2, a key protein involved in DNA replication and cell cycle regulation. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the ORC2 protein, researchers can easily detect and analyze its expression in a variety of cell types, making it an essential component for studies in molecular biology and cancer research.ORC2, part of the Origin Recognition Complex, plays a crucial role in recognizing and binding to DNA replication origins, initiating the replication process.
Dysfunction of ORC2 can lead to genomic instability and contribute to the development of cancer and other genetic disorders. By understanding the function and regulation of ORC2, researchers can gain valuable insights into the mechanisms of DNA replication and potential targets for therapeutic interventions in cancer treatment.Overall, the ORC2 Polyclonal Antibody is a reliable tool for researchers investigating the role of ORC2 in DNA replication and cell proliferation, providing valuable data for advancing our understanding of cancer biology and genetic diseases.
Product Name:
ORC2 Rabbit Polyclonal Antibody
SKU:
CAB15697
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 458-577 of human ORC2 (NP_006181.1).
The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. This protein forms a core complex with ORC3, -4, and -5. It also interacts with CDC45 and MCM10, which are proteins known to be important for the initiation of DNA replication. This protein has been demonstrated to specifically associate with the origin of replication of Epstein-Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication. Alternatively spliced transcript variants have been found, one of which is a nonsense-mediated mRNA decay candidate.
Purification Method:
Affinity purification
Gene ID:
4999
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ORC2 Rabbit pAb (CAB15697) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
