The OPN4 Polyclonal Antibody (CAB16289) is a powerful tool for research involving OPN4, a protein involved in regulating circadian rhythms and light sensitivity. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the OPN4 protein, this antibody allows for the detection and analysis of OPN4 in various cell types, making it an ideal choice for studies in neuroscience, chronobiology, and vision research.OPN4, also known as melanopsin, is a photopigment found in a specialized subset of retinal ganglion cells that play a crucial role in non-image-forming visual functions, such as regulating the sleep-wake cycle and pupillary light reflex.
Understanding the function and regulation of OPN4 is essential for unraveling the mechanisms underlying circadian rhythms, light perception, and their impact on various physiological processes. Research into OPN4 may provide insights into clinical conditions related to disrupted circadian rhythms, such as insomnia, mood disorders, and seasonal affective disorder.
Product Name:
OPN4 Rabbit Polyclonal Antibody
SKU:
CAB16289
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 365-489 of human OPN4 (NP_001025186.1).
Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. This gene encodes a photoreceptive opsin protein that is expressed within the ganglion and amacrine cell layers of the retina. In mouse, retinal ganglion cell axons expressing this gene projected to the suprachiasmatic nucleus and other brain nuclei involved in circadian photoentrainment. In mouse, this protein is coupled to a transient receptor potential (TRP) ion channel through a G protein signaling pathway and produces a physiologic light response via membrane depolarization and increased intracellular calcium. The protein functions as a sensory photopigment and may also have photoisomerase activity. Experiments with knockout mice indicate that this gene attenuates, but does not abolish, photoentrainment. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
94233
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from U-251MG cells, using OPN4 Rabbit pAb (CAB16289) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
