The OPN1SW Antibody (PAC054358) is a specific and sensitive tool designed for research involving the OPN1SW protein, also known as the short-wave-sensitive opsin 1 found in the human eye. This rabbit polyclonal antibody is highly reactive with human samples and is validated for use in various applications, particularly in immunohistochemistry and western blotting.OPN1SW is a crucial protein involved in color vision and is responsible for detecting blue light. Dysfunction of OPN1SW has been linked to various vision disorders, making it a significant target for research in the field of ophthalmology and neuroscience.
This antibody binds specifically to OPN1SW, allowing for the detection and analysis of its expression in different cell types and tissues, providing valuable insights for studies on vision-related disorders and retinal diseases.Understanding the function and regulation of OPN1SW is essential for advancing our knowledge of color vision and developing new strategies for the treatment of visual impairments. The OPN1SW Antibody serves as a vital tool for researchers investigating the role of OPN1SW in vision processes and exploring potential therapeutic interventions for vision disorders.
Western Blot. Positive WB detected in: U87 whole cell lysate. All lanes: OPN1SW antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 40 kDa. Observed band size: 40 kDa.
Immunofluorescence staining of U251 cells with PACO54358 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.
OPN1SW: Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal. Defects in OPN1SW are the cause of tritan color blindness (CBT). Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1Chromosomal Location of Human Ortholog: 7q32.1Cellular Component: integral to plasma membraneMolecular Function: G-protein coupled receptor activity; photoreceptor activity; receptor activityBiological Process: G-protein coupled receptor protein signaling pathway; phototransduction, visible light; visual perception; phototransduction; retinoid metabolic process; signal transduction; protein-chromophore linkageDisease: Tritanopia
UniProt Protein Details:
NCBI Summary:
This gene belongs to the G-protein coupled receptor 1 family, opsin subfamily. It encodes the blue cone pigment gene which is one of three types of cone photoreceptors responsible for normal color vision. Defects in this gene are the cause of tritan color blindness (tritanopia). Affected individuals lack blue and yellow sensory mechanisms while retaining those for red and green. Defective blue vision is characteristic. [provided by RefSeq, Jul 2008]
