The OGT Polyclonal Antibody (CAB1990) is a valuable tool for researchers studying OGT, a key enzyme involved in the addition of O-GlcNAc to proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity for human samples, making it an excellent choice for Western blot applications. By binding to the OGT protein, this antibody allows for precise detection and analysis in various cell types, enabling in-depth studies in glycobiology and epigenetics.OGT, or O-GlcNAc transferase, plays a crucial role in post-translational modification processes, affecting protein stability, activity, and localization.
Dysregulation of OGT has been implicated in various diseases, including cancer, diabetes, and neurodegenerative disorders. Therefore, investigating the function and regulation of OGT is essential for developing targeted therapies and understanding the underlying mechanisms of these conditions.Overall, the OGT Polyclonal Antibody (CAB1990) provides researchers with a powerful tool to delve into the intricate world of O-GlcNAcylation and its impact on cellular processes, opening up new avenues for therapeutic interventions and biomarker discovery.
Product Name:
OGT Rabbit Polyclonal Antibody
SKU:
CAB1990
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 510-699 of human OGT (NP_858058.1).
This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
8473
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using OGT Rabbit pAb (CAB1990) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
