Anti-OGFR Antibody (CAB7074)

Antibody Name:	Anti-OGFR Antibody
Antibody SKU:	CAB7074
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human OGFR (NP_031372.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-431, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human OGFR (NP_031372.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDDP DCDS TWEE DEED AEDA EDED CEDG EAAG ARDA DAGD EDEE SEEP RAAR PSSF QSRM TGSR NWRA TRDM CRYR HNYP DLVE RDCN GDTP NLSF YRNE IRFL PNGC FIED ILQN WTDN YDLL EDNH SYIQ WLFP LREP GVNW HAKP LTLR EVEV FKSS QEIQ ERLV RAYE LMLG FYGI RLED RGTG TVGR AQNY QKRF QNLN WRSH NNLR ITRI LKSL GELG LEHF QAPL VRFF LEET LVRR ELPG VRQS ALDY FMFA VRCR HQRR QLVH FAWE HFRP
Gene ID:	11054
Uniprot:	Q9NZT2
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	71kDa/73kDa
Observed MW:	110kDa

Synonyms:

OGFR

Background:

The protein encoded by this gene is a receptor for opioid growth factor (OGF), also known as [Met(5)]-enkephalin. OGF is a negative regulator of cell proliferation and tissue organization in a variety of processes. The encoded unbound receptor for OGF has been localized to the outer nuclear envelope, where it binds OGF and is translocated into the nucleus. The coding sequence of this gene contains a polymorphic region of 60 nt tandem imperfect repeat units. Several transcripts containing between zero and eight repeat units have been reported.

UniProt Protein Function:	OGFR: Receptor for opioid growth factor (OGF), also known as Met-enkephalin. Seems to be involved in growth regulation. Belongs to the opioid growth factor receptor family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Receptor, misc. Chromosomal Location of Human Ortholog: 20q13.3 Cellular Component: membrane; cytoplasm; nucleus Molecular Function:opioid receptor activity Biological Process: regulation of cell growth
NCBI Summary:	The protein encoded by this gene is a receptor for opioid growth factor (OGF), also known as [Met(5)]-enkephalin. OGF is a negative regulator of cell proliferation and tissue organization in a variety of processes. The encoded unbound receptor for OGF has been localized to the outer nuclear envelope, where it binds OGF and is translocated into the nucleus. The coding sequence of this gene contains a polymorphic region of 60 nt tandem imperfect repeat units. Several transcripts containing between zero and eight repeat units have been reported. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NZT2
NCBI GenInfo Identifier:	146331047
NCBI Gene ID:	11054
NCBI Accession:	Q9NZT2.3
UniProt Secondary Accession:	Q9NZT2,O96029, Q4VXW5, Q96CM2, Q9BQW1, Q9H4H0, Q9H7J5 Q9NZT3, Q9NZT4,
UniProt Related Accession:	Q9NZT2
Molecular Weight:	677
NCBI Full Name:	Opioid growth factor receptor
NCBI Synonym Full Names:	opioid growth factor receptor
NCBI Official Symbol:	OGFR
NCBI Protein Information:	opioid growth factor receptor; protein 7-60; zeta-type opioid receptor
UniProt Protein Name:	Opioid growth factor receptor
UniProt Synonym Protein Names:	Protein 7-60; Zeta-type opioid receptor
Protein Family:	Opioid growth factor receptor
UniProt Gene Name:	OGFR
UniProt Entry Name:	OGFR_HUMAN

	Western blot analysis of extracts of various cell lines, using OGFR antibody (CAB7074) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human stomach using OGFR antibody (CAB7074) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using OGFR antibody (CAB7074) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse stomach using OGFR antibody (CAB7074) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF7 cells using OGFR antibody (CAB7074). Blue: DAPI for nuclear staining.

Antibodies

OGFR Antibody (PACO02389)

OGFR Antibody (PACO10998)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)