The OGDH Polyclonal Antibody (CAB12477) is a valuable tool for researchers studying OGDH, a key enzyme in the tricarboxylic acid cycle involved in energy metabolism. This antibody, produced in rabbits, is highly specific to human samples and is validated for use in Western blot applications. By targeting the OGDH protein, this antibody enables accurate detection and analysis in various cell types, making it ideal for research in metabolism, energy production, and related disorders.
OGDH, also known as 2-oxoglutarate dehydrogenase, plays a crucial role in converting 2-oxoglutarate into succinyl-CoA, a critical step in the TCA cycle for energy production. Dysregulation of OGDH has been implicated in metabolic disorders, neurodegenerative diseases, and cancer. Studying the activity of OGDH using this antibody is essential for understanding its role in these conditions and developing potential therapeutic interventions.
Product Name:
OGDH Rabbit Polyclonal Antibody
SKU:
CAB12477
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 148-427 of human OGDH (NP_001003941.1).
This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
4967
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using OGDH Rabbit pAb (CAB12477) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
