The OGDH Monoclonal Antibody is a key tool for researchers studying OGDH, an enzyme involved in the tricarboxylic acid cycle and energy metabolism. This antibody, produced in mouse, has been rigorously validated for use in various applications, including immunohistochemistry and ELISA, and shows high specificity for OGDH protein detection.OGDH plays a vital role in cellular energy production and has been implicated in various diseases, including metabolic disorders and cancer.
Research utilizing the OGDH Monoclonal Antibody can provide insights into the function and regulation of OGDH, offering potential targets for therapeutic intervention in metabolic-related conditions. This antibody is essential for investigating the role of OGDH in cellular metabolism and disease pathogenesis.
Product Name:
OGDH Monoclonal Antibody
SKU:
CAB22163
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 869-1023 of human OGDH (NP_002532.2).
This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
4967
Clone Number:
ARC55379
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using OGDH Rabbit mAb (CAB22163) at1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
