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Ocrelizumab ELISA Kit (SBRS2022)

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SKU:
SBRS2022
Product Type:
ELISA Kit
Size:
96T
ELISA Type:
Sandwich ELISA
Reactivity:
Human
$2,399

Description

system_update_altDatasheet
Product Name: Ocrelizumab ELISA Kit
Product Code: SBRS2022
Size: 96T
Alias: Ranibizumab
Detection method: Sandwich ELISA
Reactivity: Human
Sample Type: Cell Culture Supernatants, Plasma, Serum
Specificity: This ELISA antibody pair detects Antibody Drug. Other targets not determined.
Storage & Stability: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
CV(%): Intra-Assay: CV<10%
Inter-Assay: CV<12%
Note: For Research Use Only

The Assay Genie Antibody Drug ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of drug antibody (DA) in serum, plasma, and cell culture supernatants.This assay employs an antibody specific for the DA coated on a 96-well plate. Standards and samples are pipetted into the wells and DA present in the sample is bound to the wells by the immobilized antibody. The wells are washed and HRP-conjugated detection antibody is added. The wells are again washed, and a TMB substrate solution is added to the wells and color develops in proportion to the amount of DA bound. The stop solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
  • Stop Solution
  • Assay Diluent
Other Materials Required
  • Microplate reader capable of measuring absorbance at 450nm
  • Precision pipettes to deliver 2 µl to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Absorbent paper
  • Distilled or deionized water
  • Log-log graph paper or computer and software for ELISA data analysis
  • Horizontal orbital microplate shaker
  • Tubes to prepare standard and sample dilutions
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well. Incubate 2 hours at room temperature.
  3. Add 100 μl prepared biotinylated antibody to each well. Incubate 1 hour at room temperature.
  4. Add 100 μl prepared Streptavidin solution. Incubate 45 minutes at room temperature.
  5. Add 100 μl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
  6. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.

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