The OCLN Polyclonal Antibody (CAB12621) is a valuable tool for researchers studying occludin, a key protein involved in tight junction formation and function in epithelial and endothelial cells. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the occludin protein, this antibody enables researchers to detect and analyze the expression levels of occludin in various cell types, making it ideal for studies related to cell junctions, barrier function, and epithelial/endothelial cell biology.Occludin is a crucial component of tight junctions, which play a pivotal role in regulating paracellular permeability and maintaining tissue integrity.
Dysregulation of occludin expression or function has been implicated in various pathological conditions, including inflammatory bowel disease, cancer metastasis, and neurological disorders. By studying occludin using this antibody, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets for intervention.In summary, the OCLN Polyclonal Antibody (CAB12621) is a reliable tool for investigating the role of occludin in physiological and pathological processes related to cell junctions and barrier function. Its specificity and sensitivity make it a valuable asset for researchers in fields such as cell biology, physiology, and pathology.
Product Name:
Occludin Rabbit Polyclonal Antibody
SKU:
CAB12621
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 350-450 of human Occludin (NP_002529.1).
This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5.
Purification Method:
Affinity purification
Gene ID:
100506658
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of 293T, using Occludin antibody (CAB12621) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
