The OAZ1 Polyclonal Antibody (CAB7444) is a valuable tool for researchers studying OAZ1, a protein involved in cell growth regulation and development. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the OAZ1 protein, this antibody enables the detection and analysis of OAZ1 in various cell types, making it well-suited for investigations in developmental biology and cancer research.OAZ1, also known as ornithine decarboxylase antizyme 1, is a critical regulator of polyamine metabolism and has been implicated in various cellular processes, including cell proliferation and differentiation.
Dysregulation of OAZ1 has been linked to diseases such as cancer, making it a promising target for therapeutic interventions. By studying the role of OAZ1 in cellular processes, researchers can gain insights into potential therapeutic strategies for cancer and other related conditions.Overall, the OAZ1 Polyclonal Antibody (CAB7444) offers a reliable and specific tool for researchers looking to investigate the function and regulation of OAZ1 in various biological contexts. Its versatility and accuracy make it a valuable asset for studies aiming to elucidate the intricate mechanisms underlying cell growth and development.
Product Name:
OAZ1 Rabbit Polyclonal Antibody
SKU:
CAB7444
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-68 of human OAZ1 (NP_004143.1).
Sequence:
MVKS SLQR ILNS HCFA REKE GDKP SATI HASR TMPL LSLH SRGG SSSE SSRV SLHC CSNP GPGP RWCS
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
AZ1; AZI; OAZ; OAZ1
Positive Sample:
MCF7,Mouse heart,Rat kidney
Conjugate:
Unconjugated
Cellular Localization:
cytoplasm, cytosol, nucleus
Calculated MW:
25kDa
Observed MW:
27kDa
The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamines. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis; thus, completing the auto-regulatory circuit. This gene encodes antizyme 1, the first member of the antizyme family, that has broad tissue distribution, and negatively regulates intracellular polyamine levels by binding to and targeting ODC for degradation, as well as inhibiting polyamine uptake. Antizyme 1 mRNA contains two potential in-frame AUGs; and studies in rat suggest that alternative use of the two translation initiation sites results in N-terminally distinct protein isoforms with different subcellular localization. Alternatively spliced transcript variants have also been noted for this gene.
Purification Method:
Affinity purification
Gene ID:
4946
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using OAZ1 antibody (CAB7444) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
