The NXF3 Polyclonal Antibody (PAC022479) is a valuable tool for researchers studying the NXF3 protein, a key player in cellular mRNA export processes. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the NXF3 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal choice for investigations in the fields of molecular biology and RNA processing.NXF3, also known as nuclear RNA export factor 3, plays a crucial role in the transport of newly synthesized messenger RNA (mRNA) from the nucleus to the cytoplasm, a fundamental step in gene expression regulation.
Dysregulation of NXF3 function has been linked to various diseases, including cancer and neurological disorders, highlighting its importance as a potential therapeutic target. Research into the mechanisms underlying NXF3 activity is essential for the development of novel treatments aimed at modulating mRNA export pathways for therapeutic benefit.
Antibody Name:
NXF3 Antibody (PACO22479)
Antibody SKU:
PACO22479
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from internal of human NXF3.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from MCF-7 cells, using NXF3 antibody.
Background:
May function as a tissue-specific nuclear mRNA export factor.
NXF3: May function as a tissue-specific nuclear mRNA export factor. Belongs to the NXF family.Chromosomal Location of Human Ortholog: Xq22Cellular Component: cytoplasm; nuclear RNA export factor complex; nucleoplasm; nucleusMolecular Function: mRNA binding; nucleotide binding; protein bindingBiological Process: mRNA export from nucleus; poly(A)+ mRNA export from nucleus
UniProt Protein Details:
NCBI Summary:
This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene has shortened LRR and ubiquitin-associated domains and its RDB is unable to bind RNA. It is located in the nucleoplasm but is not associated with either the nuclear envelope or the nucleolus. [provided by RefSeq, Jul 2008]
