The NXF1 Polyclonal Antibody (CAB5907) is a research tool designed for studying NXF1, a protein involved in mRNA nuclear export. This rabbit polyclonal antibody is highly reactive with human samples and has been validated for use in various applications such as Western blot and immunofluorescence. By specifically binding to NXF1, researchers can detect and analyze its presence in different cell types, making it an ideal tool for investigations in molecular biology and gene expression studies.
NXF1 is a key player in the process of mRNA transport from the nucleus to the cytoplasm, essential for gene expression and regulation. Dysregulation of NXF1 has been implicated in various diseases, including cancer and neurodegenerative disorders. By understanding the function and localization of NXF1, researchers can uncover important insights into cellular processes and potentially develop novel therapeutic approaches for targeting diseases associated with mRNA export dysfunction.
Product Name:
NXF1 Rabbit Polyclonal Antibody
SKU:
CAB5907
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human NXF1 (NP_006353.2).
This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
10482
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NXF1 Rabbit pAb (CAB5907) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
