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NUP98 Rabbit Polyclonal Antibody (CAB0530)

SKU:
CAB0530
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
  • Immunology Antibodies 1 Anti-NUP98 Antibody CAB0530
  • Immunology Antibodies 1 Anti-NUP98 Antibody CAB0530
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:NUP98 Rabbit Polyclonal Antibody
SKU:CAB0530
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 621-920 of human NUP98 (NP_624357.1).
Sequence:SKPV DENH QQDG DEDS LVSH FYTN PIAK PIPQ TPES AGNK HSNS NSVD DTIV ALNM RAAL RNGL EGSS EETS FHDE SLQD DREE IENN SYHM HPAG IILT KVGY YTIP SMDD LAKI TNEK GECI VSDF TIGR KGYG SIYF EGDV NLTN LNLD DIVH IRRK EVVV YLDD NQKP PVGE GLNR KAEV TLDG VWPT DKTS RCLI KSPD RLAD INYE GRLE AVSR KQGA QFKE YRPE TGSW VFKV SHFS KYGL QDSD EEEE EHPS KTST KKLK TAPL PPAS QTTP LQMA LNGK PAPP PQVE KKGQ
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:1000 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:ADIR2; NUP96; NUP196; Nup98-96; NUP98
Positive Sample:A-549,LO2,HeLa,Jurkat,HL-60
Conjugate:Unconjugated
Cellular Localization:Nucleoplasmic side, Nucleus, Nucleus membrane, Peripheral membrane protein, nuclear pore complex.
Calculated MW:198kDa
Observed MW:105kDa

Nuclear pore complexes (NPCs) regulate the transport of macromolecules between the nucleus and cytoplasm, and are composed of many polypeptide subunits, many of which belong to the nucleoporin family. This gene belongs to the nucleoporin gene family and encodes a 186 kDa precursor protein that undergoes autoproteolytic cleavage to generate a 98 kDa nucleoporin and 96 kDa nucleoporin. The 98 kDa nucleoporin contains a Gly-Leu-Phe-Gly (GLGF) repeat domain and participates in many cellular processes, including nuclear import, nuclear export, mitotic progression, and regulation of gene expression. The 96 kDa nucleoporin is a scaffold component of the NPC. Proteolytic cleavage is important for targeting of the proteins to the NPC. Translocations between this gene and many other partner genes have been observed in different leukemias. Rearrangements typically result in chimeras with the N-terminal GLGF domain of this gene to the C-terminus of the partner gene. Alternative splicing results in multiple transcript variants encoding different isoforms, at least two of which are proteolytically processed. Some variants lack the region that encodes the 96 kDa nucleoporin.

Purification Method:Affinity purification
Gene ID:4928
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using NUP98 antibody (CAB0530) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

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