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NUP98 Monoclonal Antibody (CAB22743)

SKU:
CAB22743
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
NHP - Monkey
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
$167 - $635
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:NUP98 Monoclonal Antibody
SKU:CAB22743
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Monkey
Immunogen:Recombinant protein of human NUP98.
Tested Applications:WB ELISA
Recommended Dilution:WB,1:2000 - 1:7000
Synonyms:ADIR2; NUP96; NUP196; Nup98-96; NUP98
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:Nucleoplasmic side, Nucleus, Nucleus membrane, Peripheral membrane protein, nuclear pore complex.
Calculated MW:198kDa
Observed MW:98kDa

Nuclear pore complexes (NPCs) regulate the transport of macromolecules between the nucleus and cytoplasm, and are composed of many polypeptide subunits, many of which belong to the nucleoporin family. This gene belongs to the nucleoporin gene family and encodes a 186 kDa precursor protein that undergoes autoproteolytic cleavage to generate a 98 kDa nucleoporin and 96 kDa nucleoporin. The 98 kDa nucleoporin contains a Gly-Leu-Phe-Gly (GLGF) repeat domain and participates in many cellular processes, including nuclear import, nuclear export, mitotic progression, and regulation of gene expression. The 96 kDa nucleoporin is a scaffold component of the NPC. Proteolytic cleavage is important for targeting of the proteins to the NPC. Translocations between this gene and many other partner genes have been observed in different leukemias. Rearrangements typically result in chimeras with the N-terminal GLGF domain of this gene to the C-terminus of the partner gene. Alternative splicing results in multiple transcript variants encoding different isoforms, at least two of which are proteolytically processed. Some variants lack the region that encodes the 96 kDa nucleoporin.

Purification Method:Affinity purification
Gene ID:4928
Clone Number:ARC58955
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of HeLa, using NUP98 antibody (CAB22743) at 1:6000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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