The NUP98 Monoclonal Antibody is a powerful tool for researchers studying NUP98, a nucleoporin protein involved in nucleocytoplasmic transport and gene regulation. This monoclonal antibody, produced using a specific hybridoma cell line, offers high specificity and sensitivity for detecting NUP98 in various cell types and tissues.NUP98 is known to play a critical role in mediating nuclear transport and gene expression, making it a key player in cellular processes such as mRNA export and gene regulation.
Dysregulation of NUP98 has been associated with various diseases, including leukemia and developmental disorders.By using the NUP98 Monoclonal Antibody in techniques such as Western blotting and immunofluorescence, researchers can gain valuable insights into the function and localization of NUP98 in different cellular contexts. This antibody is an essential tool for studies focusing on nucleoporins, nuclear transport mechanisms, and gene regulation.
Nuclear pore complexes (NPCs) regulate the transport of macromolecules between the nucleus and cytoplasm, and are composed of many polypeptide subunits, many of which belong to the nucleoporin family. This gene belongs to the nucleoporin gene family and encodes a 186 kDa precursor protein that undergoes autoproteolytic cleavage to generate a 98 kDa nucleoporin and 96 kDa nucleoporin. The 98 kDa nucleoporin contains a Gly-Leu-Phe-Gly (GLGF) repeat domain and participates in many cellular processes, including nuclear import, nuclear export, mitotic progression, and regulation of gene expression. The 96 kDa nucleoporin is a scaffold component of the NPC. Proteolytic cleavage is important for targeting of the proteins to the NPC. Translocations between this gene and many other partner genes have been observed in different leukemias. Rearrangements typically result in chimeras with the N-terminal GLGF domain of this gene to the C-terminus of the partner gene. Alternative splicing results in multiple transcript variants encoding different isoforms, at least two of which are proteolytically processed. Some variants lack the region that encodes the 96 kDa nucleoporin.
Purification Method:
Affinity purification
Gene ID:
4928
Clone Number:
ARC58955
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of HeLa, using NUP98 antibody (CAB22743) at 1:6000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
