The NUP50 Polyclonal Antibody (CAB9441) is a vital tool for research involving nucleoporin NUP50, a protein involved in nucleocytoplasmic transport and nuclear pore complex assembly. This antibody, produced in rabbits, has high reactivity with human samples and is validated for use in various applications, including Western blotting.NUP50 is an essential component of the nuclear pore complex, playing a crucial role in the transportation of macromolecules between the nucleus and cytoplasm. Its function in cellular trafficking makes it a key player in gene expression regulation and protein localization.
Research into NUP50's involvement in these processes can provide insights into diseases like cancer, viral infections, and neurological disorders.By utilizing the NUP50 Polyclonal Antibody, researchers can detect and analyze NUP50 protein levels in different cell types, facilitating studies in cell biology, molecular biology, and genetics. Understanding the role of NUP50 in nucleocytoplasmic transport and nuclear pore complex function is essential for advancements in drug discovery, biomarker identification, and therapeutic development.
Product Name:
NUP50 Rabbit Polyclonal Antibody
SKU:
CAB9441
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 340-468 of human NUP50 (NP_009103.2).
Sequence:
ENDE PPKV VVTE VKEE DAFY SKKC KLFY KKDN EFKE KGIG TLHL KPTA NQKT QLLV RADT NLGN ILLN VLIP PNMP CTRT GKNN VLIV CVPN PPID EKNA TMPV TMLI RVKT SEDA DELH KILL EKKD A
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
10762
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NUP50 Rabbit pAb (CAB9441) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
