The NUDT15 Polyclonal Antibody (CAB14141) is a valuable tool for researchers studying NUDT15, a nucleoside diphosphate-linked moiety X motif 15 protein involved in the metabolism of nucleotides and chemotherapy drug resistance. This rabbit-derived antibody is highly specific to human samples and is suitable for use in Western blot applications. By binding to the NUDT15 protein, this antibody enables the detection and analysis of NUDT15 in various cell types, making it a valuable asset for studies in oncology and pharmacology.
NUDT15 is known for its role in detoxifying metabolites of anticancer and antiviral nucleoside analogs, making it a key player in drug metabolism and response. Research on NUDT15 is particularly important in understanding chemotherapy resistance and optimizing treatment strategies for cancer patients. By investigating the function and regulation of NUDT15, researchers can potentially identify novel targets for overcoming drug resistance and improving patient outcomes in oncology.
Product Name:
NUDT15 Rabbit Polyclonal Antibody
SKU:
CAB14141
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human NUDT15 (NP_060753.1).
This gene encodes an enzyme that belongs to the Nudix hydrolase superfamily. Members of this superfamily catalyze the hydrolysis of nucleoside diphosphates, including substrates like 8-oxo-dGTP, which are a result of oxidative damage, and can induce base mispairing during DNA replication, causing transversions. The encoded enzyme is a negative regulator of thiopurine activation and toxicity. Mutations in this gene result in poor metabolism of thiopurines, and are associated with thiopurine-induced early leukopenia. Multiple pseudogenes of this gene have been identified.
Purification Method:
Affinity purification
Gene ID:
55270
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of rat spleen, using NUDT15 antibody (CAB14141) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
