The NUDT1 Polyclonal Antibody (CAB5474) is a valuable tool for researchers studying the NUDT1 protein, which plays a crucial role in DNA repair and maintenance. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the NUDT1 protein, this antibody enables precise detection and analysis in various cell types, making it ideal for investigations in fields such as genomics and cancer research.NUDT1, also known as nudix hydrolase 1, is involved in DNA damage repair mechanisms and helps maintain genomic stability. Its role in DNA metabolism and repair pathways makes it a key player in protecting cells from mutations and promoting genomic integrity.
Research into the function of NUDT1 is essential for understanding processes like DNA replication and the response to DNA damage, which have implications for cancer development and therapeutic strategies.The NUDT1 Polyclonal Antibody is a valuable tool for researchers seeking to explore the intricate mechanisms of DNA repair and maintenance, and its versatility in various applications makes it a valuable asset for advancing scientific knowledge in the fields of molecular biology and oncology.
Product Name:
MTH1 Rabbit Polyclonal Antibody
SKU:
CAB5474
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human MTH1 (NP_945192.1).
Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A rare single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described.
Purification Method:
Affinity purification
Gene ID:
4521
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MTH1 antibody (CAB5474) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
