The Nucleolin Polyclonal Antibody (CAB5904) is a valuable tool for researchers studying nucleolin, a multifunctional protein involved in various cellular processes. This antibody is raised in rabbits and shows high reactivity with human samples, making it suitable for a wide range of research applications.Nucleolin is known for its role in regulating gene expression, ribosome biogenesis, and nucleocytoplasmic transport, making it a key player in cell growth and proliferation. The Nucleolin Polyclonal Antibody binds specifically to nucleolin protein, allowing for its detection and analysis in different cell types.
Researchers can use this antibody in techniques like Western blotting to investigate nucleolin expression levels and localization within cells. With its versatility and reliability, the Nucleolin Polyclonal Antibody is an essential tool for studies in molecular biology, cancer research, and other fields where nucleolin's functions are of interest.
Product Name:
NCL Rabbit Polyclonal Antibody
SKU:
CAB5904
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 300-466 of human NCL (NP_005372.2).
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
Purification Method:
Affinity purification
Gene ID:
4691
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NCL Rabbit pAb (CAB5904) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
