NUCB1 Polyclonal Antibody (CAB21817)
- SKU:
- CAB21817
Frequently bought together:
Description
| Product Name: | NUCB1 Polyclonal Antibody |
| SKU: | CAB21817 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 302-461 of human NUCB1 (NP_006175.2). |
| Sequence: | KNVD TNQD RLVT LEEF LAST QRKE FGDT GEGW ETVE MHPA YTEE ELRR FEEE LAAR EAEL NAKA QRLS QETE ALGR SQGR LEAQ KREL QQAV LHME QRKQ QQQQ QQGH KAPA AHPE GQLK FHPD TDDV PVPA PAGD QKEV DTSE KKLL ERLP EVEV PQHL |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:2000 - 1:5000 |
| Synonyms: | NUC; CALNUC; NUCB1 |
| Positive Sample: | A549,Hep G2,293T,BxPC-3 |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cytoplasm, Golgi apparatus, Lumenal side, Peripheral membrane protein, cis-Golgi network membrane. |
| Calculated MW: | 54kDa |
| Observed MW: | 60kDa |
This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events.
| Purification Method: | Affinity purification |
| Gene ID: | 4924 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using NUCB1 Rabbit pAb (CAB21817) at 1:3000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 90s. |
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