The NT5C1A Polyclonal Antibody (PAC016796) is a valuable tool for researchers studying NT5C1A, a key enzyme involved in nucleotide metabolism. This antibody, generated in rabbits, demonstrates high specificity towards human samples and is optimized for Western blot applications. By binding to the NT5C1A protein, this antibody allows for the detection and analysis of NT5C1A in a variety of cell types, making it a versatile tool for studies in molecular biology and cancer research.NT5C1A plays a crucial role in maintaining nucleotide balance within cells, making it essential for processes such as DNA repair and cell proliferation.
Dysregulation of NT5C1A has been linked to various diseases, including cancer and autoimmune disorders, highlighting the importance of studying this enzyme in disease pathogenesis. By understanding the function of NT5C1A, researchers can develop targeted therapies that modulate nucleotide metabolism for the treatment of these conditions.
Antibody Name:
NT5C1A Antibody (PACO16796)
Antibody SKU:
PACO16796
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:50-1:200
Species Reactivity:
Human, Mouse
Immunogen:
Fusion protein of human NT5C1A
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using PACO16796(NT5C1A Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using PACO16796(NT5C1A Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200).
Background:
Cytosolic nucleotidases, such as NT5C1A, dephosphorylate nucleoside monophosphates. Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides and has a broad substrate specificity. Helps to regulate adenosine levels in heart during ischemia and hypoxia.
Synonyms:
5'-nucleotidase, cytosolic IA
UniProt Protein Function:
NT5C1A: Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides and has a broad substrate specificity. Helps to regulate adenosine levels in heart during ischemia and hypoxia. Belongs to the 5'-nucleotidase type 3 family.Protein type: Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 3.1.3.5; Phosphatase (non-protein)Chromosomal Location of Human Ortholog: 1p34.3-p33Cellular Component: cytosolMolecular Function: 5'-nucleotidase activity; magnesium ion binding; nucleotide bindingBiological Process: adenosine metabolic process; dephosphorylation; nucleobase, nucleoside and nucleotide metabolic process; nucleoside metabolic process; purine base metabolic process; purine nucleoside monophosphate catabolic process; purine nucleotide catabolic process; pyrimidine base metabolic process; pyrimidine nucleoside catabolic process
UniProt Protein Details:
NCBI Summary:
Cytosolic nucleotidases, such as NT5C1A, dephosphorylate nucleoside monophosphates (Hunsucker et al., 2001 [PubMed 11133996]).[supplied by OMIM, Mar 2008]
