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NSFL1C Rabbit Polyclonal Antibody (CAB6677)

SKU:
CAB6677
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 10 Anti-NSFL1C Antibody CAB6677
  • Cell Biology Antibodies 10 Anti-NSFL1C Antibody CAB6677
  • Cell Biology Antibodies 10 Anti-NSFL1C Antibody CAB6677
$167 - $503
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:NSFL1C Rabbit Polyclonal Antibody
SKU:CAB6677
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human NSFL1C (NP_057227.2).
Sequence:MAAE RQEA LREF VAVT GAEE DRAR FFLE SAGW DLQI ALAS FYED GGDE DIVT ISQA TPSS VSRG TAPS DNRV TSFR DLIH DQDE DEEE EEGQ RFYA GGSE RSGQ QIVG PPRK KSPN ELVD DLFK GAKE HGAV AVER VTKS PGET SKPR PFAG GGYR LGAA PEEE SAYV AGEK RQHS SQDV HVVL K
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:P47; UBX1; UBXD10; UBXN2C; dJ776F14.1; NSFL1C
Positive Sample:293T,U-251MG,Mouse kidney,Mouse brain,Rat brain,Rat kidney
Conjugate:Unconjugated
Cellular Localization:Chromosome, Golgi apparatus, Golgi stack, Nucleus.
Calculated MW:41kDa
Observed MW:41kDa

N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8.

Purification Method:Affinity purification
Gene ID:55968
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using NSFL1C Rabbit pAb (CAB6677) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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