The NSDHL Polyclonal Antibody (CAB16893) is a valuable tool for research involving the NAD(P)H steroid dehydrogenase-like protein (NSDHL), an enzyme involved in cholesterol biosynthesis and lipid metabolism. The antibody, produced in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications. By targeting NSDHL, this antibody enables researchers to detect and analyze the protein in various cell types, making it ideal for studies in lipid metabolism, cholesterol homeostasis, and related diseases.NSDHL is a key enzyme in the mevalonate pathway, which plays a crucial role in the production of cholesterol, steroid hormones, and other important molecules in the body.
Dysregulation of NSDHL activity has been linked to various metabolic disorders, including Smith-Lemli-Opitz syndrome and CHILD syndrome. Research into NSDHL function is essential for understanding the underlying mechanisms of these diseases and for developing potential therapeutic strategies that target cholesterol metabolism.Overall, the NSDHL Polyclonal Antibody (CAB16893) is a valuable tool for researchers studying lipid metabolism, cholesterol biosynthesis, and related metabolic disorders. Its high specificity and sensitivity make it an excellent choice for Western blotting experiments aimed at elucidating the role of NSDHL in health and disease.
Product Name:
[KO Validated] NSDHL Rabbit Polyclonal Antibody
SKU:
CAB16893
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human NSDHL (NP_057006.1).
The protein encoded by this gene is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
50814
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type(WT) and NSDHL knockout (KO) 293T(KO) cells, using [KO Validated] NSDHL Rabbit pAb (CAB16893) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
