The NRF1 Rabbit Monoclonal Antibody (CAB3252) is a high-quality antibody designed for research involving the NRF1 protein, a transcription factor that plays a key role in regulating the expression of genes involved in mitochondrial function and oxidative stress response. This antibody, generated in rabbits, is highly specific and reactive with human samples, making it an excellent tool for studying NRF1 in various biological systems. Validated for use in Western blot applications, the NRF1 Rabbit Monoclonal Antibody binds specifically to the NRF1 protein, allowing for accurate detection and analysis in a variety of cell types.
Given the crucial role of NRF1 in maintaining cellular homeostasis and response to stress, this antibody is particularly valuable for studies in research areas such as cell biology, metabolism, and oxidative stress. Understanding the function and regulation of NRF1 can provide insights into a wide range of diseases and conditions, including cancer, neurodegenerative disorders, and metabolic diseases. By targeting NRF1, researchers can potentially develop novel therapeutic strategies for addressing these health challenges.
Product Name:
NRF1 Rabbit Monoclonal Antibody
SKU:
CAB3252
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human NRF1 (Q16656).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,5μg antibody for 10μg-15μg of ChromatinCUT&Tag, 10⁵ cells /1 μg
Synonyms:
ALPHA-PAL; NRF1
Positive Sample:
NIH/3T3,Mouse lung,Rat testis,HeLa,293T,HepG2
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
54kDa
Observed MW:
68kDa
This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1.
Purification Method:
Affinity purification
Gene ID:
4899
Clone Number:
ARC0768
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina(RK20265) from 10⁵ HepG2 cells with 1 μg NRF1 Rabbit mAb (CAB3252), along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of NRF1 in representative gene loci (LINS1), as shown in figure.
