The NRF1 Polyclonal Antibody (CAB5547) is a crucial tool for researchers studying NRF1, a transcription factor involved in the regulation of cellular responses to oxidative stress and mitochondrial biogenesis. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the NRF1 protein, this antibody enables precise detection and analysis, making it ideal for studies in molecular biology and aging research.NRF1, also known as nuclear respiratory factor 1, plays a key role in controlling the expression of genes involved in mitochondrial function and antioxidant defense.
Dysregulation of NRF1 has been linked to various diseases, including neurodegenerative disorders and metabolic syndromes. Understanding the mechanisms by which NRF1 operates is essential for developing interventions aimed at maintaining cellular health and combating age-related decline.With its high reactivity and specificity, the NRF1 Polyclonal Antibody (CAB5547) is a valuable tool for researchers seeking to unravel the complexities of NRF1 biology and its implications for human health.
Product Name:
NRF1 Rabbit Polyclonal Antibody
SKU:
CAB5547
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 240-449 of human NRF1 (NP_001035199.1).
This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1.
Purification Method:
Affinity purification
Gene ID:
4899
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using NRF1 Rabbit pAb (CAB5547) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
