Anti-NUR77 Antibody (CAB16264)
- SKU:
- CAB16264
- Product Type:
- Antibody
- Reactivity:
- Human
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
Anti-NUR77 Antibody (CAB16264)
The NR4A1 Polyclonal Antibody (CAB16264) is a valuable tool for researchers studying the NR4A1 protein, a key transcription factor involved in various cellular processes including apoptosis, inflammation, and metabolism. This antibody, produced in rabbits, is highly specific to human NR4A1 samples and is validated for use in Western blot applications. By binding to the NR4A1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential component for studies in fields such as immunology and cancer research.
NR4A1, also known as Nur77 or NGFI-B, plays a vital role in regulating gene expression and cellular functions in response to various stimuli. Its involvement in pathways that control cell survival, proliferation, and inflammation make it a crucial target for investigating diseases such as cancer, autoimmune disorders, and metabolic disorders. Understanding the role of NR4A1 can provide insights into potential therapeutic strategies that target this protein for the treatment of these conditions.
| Antibody Name: | Anti-NUR77 Antibody |
| Antibody SKU: | CAB16264 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human NUR77 (NP_775180.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Rat |
| Positive Samples: | Rat lung |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human NUR77 (NP_775180.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PSLA QSPL KLFP SQAT HQLG EGES YSMP TAFP GLAP TSPH LEGS GILD TPVT STKA RSGA PGGS EGRC AVCG DNAS CQHY GVRT CEGC KGFF KRTV QKNA K |
| Gene ID: | 3164 |
| Uniprot: | P22736 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 33kDa/64kDa/65kDa |
| Observed MW: | 70kDa |
| Synonyms: | NR4A1, GFRP1, HMR, N10, NAK-1, NGFIB, NP10, NUR77, TR3 |
| Background: | This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | Nur77: an orphan nuclear receptor and immediate-early gene that regulates cellular proliferation, apoptosis, inflammation, and glucose metabolism. Induced by exercise in muscle and is a functional regulator of glucose metabolism in skeletal muscle. Its level decreases in the muscle of obese insulin-resistant men. Acts concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. A mediator of TCR-directed thymocyte apoptosis. TCR-signaling induces a FAIM/Akt/Nur77 signaling pathway that is critical for modulating apoptosis in developing thymocytes. A physiological substrate of the MEK-ERK-RSK cascade that modulates nuclear export and intracellular translocation during T cell death. Binds DNA as a monomer. Interacts with GADD45GIP1. Overexpression of Nur77 induces the expression of both p300 and HDAC1. Acetylation by p300 and HDAC1 may regulate the rapid turnover of Nur77 protein. |
| UniProt Protein Details: | Protein type:Nuclear receptor; Apoptosis; DNA-binding Chromosomal Location of Human Ortholog: 12q13 Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nucleus Molecular Function:protein binding; ligand-dependent nuclear receptor activity; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity Biological Process: epidermal growth factor receptor signaling pathway; transcription initiation from RNA polymerase II promoter; phosphoinositide-mediated signaling; intracellular receptor-mediated signaling pathway; fibroblast growth factor receptor signaling pathway; cell migration during sprouting angiogenesis; nerve growth factor receptor signaling pathway; innate immune response; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; signal transduction |
| NCBI Summary: | This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011] |
| UniProt Code: | P22736 |
| NCBI GenInfo Identifier: | 127819 |
| NCBI Gene ID: | 3164 |
| NCBI Accession: | P22736.1 |
| UniProt Secondary Accession: | P22736,Q15627, B4DML7, |
| UniProt Related Accession: | P22736 |
| Molecular Weight: | 64,463 Da |
| NCBI Full Name: | Nuclear receptor subfamily 4 group A member 1 |
| NCBI Synonym Full Names: | nuclear receptor subfamily 4, group A, member 1 |
| NCBI Official Symbol: | NR4A1Â Â |
| NCBI Official Synonym Symbols: | HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77Â Â |
| NCBI Protein Information: | nuclear receptor subfamily 4 group A member 1; ST-59; hormone receptor; TR3 orphan receptor; steroid receptor TR3; testicular receptor 3; early response protein NAK1; orphan nuclear receptor HMR; orphan nuclear receptor TR3; nuclear hormone receptor NUR/77; growth factor-inducible nuclear protein N10; nerve growth factor IB nuclear receptor variant 1 |
| UniProt Protein Name: | Nuclear receptor subfamily 4 group A member 1 |
| UniProt Synonym Protein Names: | Early response protein NAK1; Nuclear hormone receptor NUR/77; Nur77; Orphan nuclear receptor HMR; Orphan nuclear receptor TR3; ST-59; Testicular receptor 3 |
| Protein Family: | Nuclear receptor subfamily |
| UniProt Gene Name: | NR4A1Â Â |
| UniProt Entry Name: | NR4A1_HUMAN |
 | Western blot analysis of extracts of rat lung, using NUR77 antibody (CAB16264) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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