The NR3C1 Polyclonal Antibody (CAB2164) is a valuable tool for researchers studying the NR3C1 protein, also known as glucocorticoid receptor (GR), which plays a pivotal role in mediating the effects of glucocorticoids on gene expression. This antibody, produced in rabbits, exhibits high specificity and reactivity with human samples, making it ideal for applications such as Western blotting.The NR3C1 protein is involved in various physiological processes, including immune response regulation, metabolism, and stress responses. Dysregulation of NR3C1 function has been linked to diseases such as inflammation, autoimmune disorders, and psychiatric disorders.
Therefore, studying the NR3C1 protein and its activity can provide crucial insights into disease mechanisms and potential therapeutic targets.By using the NR3C1 Polyclonal Antibody (CAB2164), researchers can effectively detect and analyze NR3C1 protein levels in different cell types and tissues, enabling a deeper understanding of its biological functions. This antibody is a valuable tool for investigations in fields such as immunology, endocrinology, and pharmacology, ultimately contributing to advancements in disease research and drug development.
This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175).
Purification Method:
Affinity purification
Gene ID:
2908
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Glucocorticoid Receptor Rabbit pAb (CAB2164) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
