The NR1I3 Rabbit Polyclonal Antibody (CAB17066) is a valuable tool for researchers studying NR1I3, a nuclear receptor that plays a key role in regulating drug metabolism and detoxification pathways. This antibody, generated in rabbits, exhibits high specificity for human samples and is validated for use in Western blot applications. By binding to the NR1I3 protein, this antibody enables precise detection and analysis in various cell types, making it ideal for investigations in pharmacology and toxicology research.
NR1I3, also known as the pregnane X receptor (PXR), is crucial for maintaining the body's response to xenobiotics and endobiotics, aiding in the clearance of potentially harmful substances. Its involvement in drug interactions and metabolism makes it a significant target for studies related to drug development, drug-drug interactions, and personalized medicine. Understanding the role of NR1I3 is essential for advancing research in pharmacogenomics and enhancing drug safety and efficacy.
Product Name:
NR1I3 Rabbit Polyclonal Antibody
SKU:
CAB17066
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 103-352 of human NR1I3 (NP_001070948.1).
This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. In addition to drug metabolism, the CAR protein is also reported to regulate genes involved in glucose metabolism, lipid metabolism, cell proliferation, and circadian clock regulation. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
9970
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NR1I3 Rabbit pAb (CAB17066) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
