The NR1I3 Polyclonal Antibody (CAB1970) is a valuable tool for researchers studying NR1I3, a nuclear receptor involved in the transcriptional regulation of genes related to drug metabolism and transport. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the NR1I3 protein, this antibody allows for the detection and analysis of NR1I3 expression in various cell types, making it ideal for studies in pharmacology and toxicology.
NR1I3, also known as the pregnane X receptor (PXR), plays a crucial role in the regulation of drug metabolism and detoxification pathways in the liver and intestine. Its involvement in mediating drug interactions and responses to xenobiotics makes it a key target for research in pharmacogenomics and personalized medicine. Understanding the function of NR1I3 is essential for developing strategies to optimize drug efficacy and minimize adverse effects in clinical settings.
Product Name:
NR1I3 Rabbit Polyclonal Antibody
SKU:
CAB1970
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 103-352 of human NR1I3 (NP_001070948.1).
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
CAR; CAR1; MB67; NR1I3
Positive Sample:
HT-29,Mouse liver,Rat liver,293T
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus, cytoskeleton.
Calculated MW:
40kDa
Observed MW:
45kDa/40kDa
This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. In addition to drug metabolism, the CAR protein is also reported to regulate genes involved in glucose metabolism, lipid metabolism, cell proliferation, and circadian clock regulation. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
9970
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NR1I3 Rabbit pAb (CAB1970) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
